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KMID : 0613820210310030338
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Journal of Life Science
2021 Volume.31 No. 3 p.338 ~ p.345
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The Protective Effect of Zizania latifolia Extract against t-BHP-induced Oxidative Stress in HepG2 Cells
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Park Se-Ho
Lee Jae-Yeul
Yang Seun-Ah
Bang Dae-Suk
Jhee Kwang-Hwan
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Abstract
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Zizania latifoliahas long been used as a tea for both edible and medicinal purposes. However, research into the use of Z. latifoliaas a high value-added edible material is lacking. In a previous study, we confirmed that tricin is the major component in Z. latifolia. In this study, we investigated the protective effect of a Z. latifoliaextract (ZLE). Toxicity tests of ZLE or tricin on HepG2 cells revealed no toxicity due to ZLEor tricin at all concentrations used. The reduction in cell viability by tert-butyl hydroperoxide (t-BHP) was suppressed by treatment with ZLE or tricin. In addition, ZLE or tricin effectively inhibited the production of reactive oxygen species (generation of hydrogen peroxide, alkoxy free radicals, and peroxyl free radicals by t-BHP) and oxidative damage. ZLE or tricin treatments also increased the protein expression of superoxide dismutase 1 (SOD1), catalase, heme oxygenase-1 (HO- 1), and nuclear factor erythroid-related factor 2 (Nrf2), which are known as antioxidant enzymes, suggesting that the protective effect of ZLE is related to activation of tricin. Taken together, the results indicate that Z. latifoliacan be developed as a functional food material for improving liver function.
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KEYWORD
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Oxidative stress, protective effect, reactive oxygen species, Zizania latifolia
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